The M Protein of SARS-CoV: Basic Structural and Immunological Properties
Identifieur interne : 005D55 ( Main/Exploration ); précédent : 005D54; suivant : 005D56The M Protein of SARS-CoV: Basic Structural and Immunological Properties
Auteurs : Yongwu Hu [République populaire de Chine] ; Jie Wen [République populaire de Chine] ; Lin Tang [République populaire de Chine] ; Haijun Zhang [République populaire de Chine] ; Xiaowei Zhang [République populaire de Chine] ; Yan Li [République populaire de Chine] ; Jing Wang [République populaire de Chine] ; Yujun Han [République populaire de Chine] ; Guoqing Li [République populaire de Chine] ; Jianping Shi [République populaire de Chine] ; Xiangjun Tian [République populaire de Chine] ; Feng Jiang [République populaire de Chine] ; Xiaoqian Zhao [République populaire de Chine] ; Jun Wang [République populaire de Chine] ; Siqi Liu [République populaire de Chine] ; Changqing Zeng [République populaire de Chine] ; Jian Wang [République populaire de Chine] ; Huanming Yang [République populaire de Chine]Source :
- Genomics, Proteomics & Bioinformatics [ 1672-0229 ] ; 2003.
Descripteurs français
- KwdFr :
- Alignement de séquences, Analyse de regroupements, Analyse de séquence d'ADN, Données de séquences moléculaires, Dosage immunologique, Mutation (génétique), Oligopeptides, Phylogénie, Protéines de la matrice virale (), Protéines de la matrice virale (génétique), Protéines de la matrice virale (immunologie), Structure tertiaire des protéines, Séquence d'acides aminés, Séquence nucléotidique, Test ELISA, Virus du SRAS (génétique).
- MESH :
- génétique : Mutation, Protéines de la matrice virale, Virus du SRAS.
- immunologie : Protéines de la matrice virale.
- Alignement de séquences, Analyse de regroupements, Analyse de séquence d'ADN, Données de séquences moléculaires, Dosage immunologique, Oligopeptides, Phylogénie, Protéines de la matrice virale, Structure tertiaire des protéines, Séquence d'acides aminés, Séquence nucléotidique, Test ELISA.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Cluster Analysis, Enzyme-Linked Immunosorbent Assay, Immunoassay, Molecular Sequence Data, Mutation (genetics), Oligopeptides, Phylogeny, Protein Structure, Tertiary, SARS Virus (genetics), Sequence Alignment, Sequence Analysis, DNA, Viral Matrix Proteins (chemistry), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
- MESH :
- chemical , chemistry : Viral Matrix Proteins.
- chemical , genetics : Viral Matrix Proteins.
- chemical , immunology : Viral Matrix Proteins.
- chemical : Oligopeptides.
- genetics : Mutation, SARS Virus.
- Amino Acid Sequence, Base Sequence, Cluster Analysis, Enzyme-Linked Immunosorbent Assay, Immunoassay, Molecular Sequence Data, Phylogeny, Protein Structure, Tertiary, Sequence Alignment, Sequence Analysis, DNA.
Abstract
We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites.
Url:
DOI: 10.1016/S1672-0229(03)01016-7
PubMed: 15626342
PubMed Central: 5172243
Affiliations:
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Le document en format XML
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Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15626342</idno><idno type="pmc">5172243</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172243</idno><idno type="RBID">PMC:5172243</idno><idno type="doi">10.1016/S1672-0229(03)01016-7</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">001022</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001022</idno><idno type="wicri:Area/Pmc/Curation">001022</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001022</idno><idno type="wicri:Area/Pmc/Checkpoint">001783</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001783</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15626342</idno><idno type="wicri:Area/PubMed/Corpus">002984</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002984</idno><idno type="wicri:Area/PubMed/Curation">002984</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002984</idno><idno type="wicri:Area/PubMed/Checkpoint">003033</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003033</idno><idno type="wicri:Area/Ncbi/Merge">000C81</idno><idno type="wicri:Area/Ncbi/Curation">000C81</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C81</idno><idno type="wicri:doubleKey">1672-0229:2003:Hu Y:the:m:protein</idno><idno type="wicri:Area/Main/Merge">006227</idno><idno type="wicri:Area/Main/Curation">005D55</idno><idno type="wicri:Area/Main/Exploration">005D55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The M Protein of SARS-CoV: Basic Structural and Immunological Properties</title><author><name sortKey="Hu, Yongwu" sort="Hu, Yongwu" uniqKey="Hu Y" first="Yongwu" last="Hu">Yongwu Hu</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Haijun" sort="Zhang, Haijun" uniqKey="Zhang H" first="Haijun" last="Zhang">Haijun Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Xiaowei" sort="Zhang, Xiaowei" uniqKey="Zhang X" first="Xiaowei" last="Zhang">Xiaowei Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff0015">College of Life Sciences, Peking University, Beijing 100871, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Han, Yujun" sort="Han, Yujun" uniqKey="Han Y" first="Yujun" last="Han">Yujun Han</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Guoqing" sort="Li, Guoqing" uniqKey="Li G" first="Guoqing" last="Li">Guoqing Li</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shi, Jianping" sort="Shi, Jianping" uniqKey="Shi J" first="Jianping" last="Shi">Jianping Shi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Jiang, Feng" sort="Jiang, Feng" uniqKey="Jiang F" first="Feng" last="Jiang">Feng Jiang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhao, Xiaoqian" sort="Zhao, Xiaoqian" uniqKey="Zhao X" first="Xiaoqian" last="Zhao">Xiaoqian Zhao</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Siqi" sort="Liu, Siqi" uniqKey="Liu S" first="Siqi" last="Liu">Siqi Liu</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author></analytic><series><title level="j">Genomics, Proteomics & Bioinformatics</title><idno type="ISSN">1672-0229</idno><idno type="eISSN">2210-3244</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cluster Analysis</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunoassay</term><term>Molecular Sequence Data</term><term>Mutation (genetics)</term><term>Oligopeptides</term><term>Phylogeny</term><term>Protein Structure, Tertiary</term><term>SARS Virus (genetics)</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Viral Matrix Proteins (chemistry)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Dosage immunologique</term><term>Mutation (génétique)</term><term>Oligopeptides</term><term>Phylogénie</term><term>Protéines de la matrice virale ()</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines de la matrice virale (immunologie)</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Test ELISA</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Oligopeptides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mutation</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mutation</term><term>Protéines de la matrice virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cluster Analysis</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunoassay</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Structure, Tertiary</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Dosage immunologique</term><term>Oligopeptides</term><term>Phylogénie</term><term>Protéines de la matrice virale</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Michael, M" uniqKey="Michael M">M. Michael</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lapps, W" uniqKey="Lapps W">W. Lapps</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fleming, J O" uniqKey="Fleming J">J.O. Fleming</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Armstrong, J" uniqKey="Armstrong J">J. Armstrong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K. Narayanan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qin, E D" uniqKey="Qin E">E.D. Qin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melen, K" uniqKey="Melen K">K. Melen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K. Narayanan</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuo, L" uniqKey="Kuo L">L. Kuo</name></author><author><name sortKey="Masters, P S" uniqKey="Masters P">P.S. Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez, I" uniqKey="Rodriguez I">I. Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lecomte, J" uniqKey="Lecomte J">J. Lecomte</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Haan, C A" uniqKey="De Haan C">C.A. de Haan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="An, S W" uniqKey="An S">S.W. An</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li><li>Zhejiang</li></region><settlement><li>Hangzhou</li><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Hu, Yongwu" sort="Hu, Yongwu" uniqKey="Hu Y" first="Yongwu" last="Hu">Yongwu Hu</name></noRegion><name sortKey="Han, Yujun" sort="Han, Yujun" uniqKey="Han Y" first="Yujun" last="Han">Yujun Han</name><name sortKey="Hu, Yongwu" sort="Hu, Yongwu" uniqKey="Hu Y" first="Yongwu" last="Hu">Yongwu Hu</name><name sortKey="Jiang, Feng" sort="Jiang, Feng" uniqKey="Jiang F" first="Feng" last="Jiang">Feng Jiang</name><name sortKey="Li, Guoqing" sort="Li, Guoqing" uniqKey="Li G" first="Guoqing" last="Li">Guoqing Li</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Liu, Siqi" sort="Liu, Siqi" uniqKey="Liu S" first="Siqi" last="Liu">Siqi Liu</name><name sortKey="Shi, Jianping" sort="Shi, Jianping" uniqKey="Shi J" first="Jianping" last="Shi">Jianping Shi</name><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name><name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name><name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name><name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name><name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name><name sortKey="Zhang, Haijun" sort="Zhang, Haijun" uniqKey="Zhang H" first="Haijun" last="Zhang">Haijun Zhang</name><name sortKey="Zhang, Xiaowei" sort="Zhang, Xiaowei" uniqKey="Zhang X" first="Xiaowei" last="Zhang">Xiaowei Zhang</name><name sortKey="Zhao, Xiaoqian" sort="Zhao, Xiaoqian" uniqKey="Zhao X" first="Xiaoqian" last="Zhao">Xiaoqian Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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